Prepare cells
Plate target cells with your current protocol. No coating step.
Research use only
HiTE™ Transduction Enhancer
Lentiviral transduction enhancer for primary T cells, NK cells, and stem cells. Add it to the vector mix.
Cell Type
(+) CD34+ HSPCs, MSCs, ESCs, Macrophages, Fibroblasts &
If your cell type isn’t listed here, reach out to us and we’ll direct you to the right product.
Size
$99
Quantity
Trial kits are excluded from the 10% promo.
Available through Fisher Scientific. For GMP grade inquiries, contact us.
Description
HiTE™ is a synthetic peptide supplied as three formulations: HiTE-T, HiTE-NK, and HiTE-SC. It is added directly to a lentiviral transduction.
- No plate coating.
- No spinoculation.
- Fits a standard transduction protocol.
- Shipped on dry ice. Store at −80°C.
Workflow
HiTE™ goes into the vector mix. The rest of the protocol stays the one you already run.
Add HiTE and vector
Mix the peptide into the lentiviral preparation and add it to the cells.
Incubate
Continue with your usual incubation and readout. Active handling stays under an hour.
Supporting data
Primary human cells at low MOI. Bars are scaled to the HiTE result in each panel. Internal testing. Results vary.
CD3+ T cells
No enhancer7.2%
Polybrene8.0%
LentiBOOST15.3%
HiTE63.0%
iPSCs
No enhancer21.2%
Polybrene16.8%
LentiBOOST23.7%
HiTE73.9%
NK cells
No enhancer0.8%
Polybrene6.0%
LentiBOOST0.7%
HiTE33.3%
1.3×Viability vs. current standard
<3Vector copy number
<1 hrActive handling
−80°CStorage
Performance data reflects internal testing under optimized conditions. Results may vary. See Terms of Service for details.
References
Peer-reviewed papers that cite HiTE™ will be listed as they are published. The technical white paper is the current reference.
Quick facts
What is in the tube?
Synthetic peptide in sterile water. Stock concentration is 400 μM. Working concentration is 80 μM for T cells, 80–100 μM for NK cells, and 40 μM for iPSCs.
How is it stored and shipped?
Store at −80°C for up to 12 months. After the first thaw, aliquot and return to −80°C. Up to 3 freeze/thaw cycles. Do not vortex. Shipped on dry ice.
What sizes can I order?
Each formulation is sold as a 1 mg trial kit (about 20 reactions, $99), 10 mg (about 200 reactions), and 50 mg (about 1,000 reactions). Trial kits are excluded from the 10% promo code.
What is it cleared for?
For research use only. Not for use in diagnostic procedures. GMP supply is handled as a separate inquiry.